Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT14G10
- UniProt No.
- Q9NP79
- NCBI Accession No.
- NP_057569
- Alternative names
- vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human VTA1 (1-307aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB,ICC/IF,FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of VTA1 in Hep3B cells. The cell was stained with ATGA0403 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: 293T cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Jurkat cell lysate
Lane 7.: K562 cell lysate
Lane 8.: LnCaP cell lysate
Lane 9.: U937 cell lysate
Lane 10.: A431 cell lysate
Lane 1.: HepG2 cell lysate
Lane 2.: 293T cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Jurkat cell lysate
Lane 7.: K562 cell lysate
Lane 8.: LnCaP cell lysate
Lane 9.: U937 cell lysate
Lane 10.: A431 cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.