Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT22E9
- UniProt No.
- O95831
- NCBI Accession No.
- NP_665811
- Alternative names
- Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, AIF, CMTX4, COWCK, COXPD6, PDCD8
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human AIFM1 (98-609aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of AIFM1 in Hep3B cells. The cell was stained with ATGA0411 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Jurkat cell lysate
Lane 2. : HeLa cell lysate
Lane 3. : Hep3B cell lysate
Lane 4. : Raji cell lysate
Lane 5. : K562 cell lysate
Lane 6. : MCF7 cell lysate
Lane 7. : CTLL2 cell lysate
Lane 1. : Jurkat cell lysate
Lane 2. : HeLa cell lysate
Lane 3. : Hep3B cell lysate
Lane 4. : Raji cell lysate
Lane 5. : K562 cell lysate
Lane 6. : MCF7 cell lysate
Lane 7. : CTLL2 cell lysate
The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Mouse heart tissue lysate
Lane 2. : Mouse liver tissue lysate
Lane 1. : Mouse heart tissue lysate
Lane 2. : Mouse liver tissue lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.