Human ALDH2 antibody

Cat No.
ATGA0450

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2C10
UniProt No.
P05091
NCBI Accession No.
NP_000681
Alternative names
Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase, mitochondrial, Ahd-5, Ahd5

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human ALDH2 (18-517aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HepG2 cell lysate
Lane 2. : Mouse liver tissue lysate
Lane 3. : Mouse lung tissue lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : ALDH2 transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.