Human Dkk-3 antibody

Cat No.
ATGA0476

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT103B5
UniProt No.
Q9UBP4
NCBI Accession No.
NP_001018067
Alternative names
Dickkopf-related protein 3 precursor, REIC, RIG

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human DKK3 (22-350aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DKK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: A549 cell lysate
Lane 5.: Mouse brain tissue lysate
Lane 6.: Mouse kidney tissue lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.