Human Sulfotransferase 2A1/SULT2A1 antibody

Cat No.
ATGA0491

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT13E10
UniProt No.
Q06520
NCBI Accession No.
NP_003158
Alternative names
Sulfotransferase family 2A member 1, ST2A1, Cytosolic sulfotransferase family 2A, Bile salt sulfotransferase, Dehydroepiandrosterone sulfotransferase, DHEA-ST, DHEA-ST8, Hydroxysteroid Sulfotransferase, HST, ST2, SULT2A3, STD

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human SULT2A1 (1-285aa) purified from E.coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of SULT2A1 in Hep3B The cell was stained with ATGA0491 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SULT2A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SULT2A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: SULT2A1 transfected 293T cell lysate
Lane 2.: 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.