NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

AT3F3

UniProt No.

Q9NS23

NCBI Accession No.

NP_009113

Alternative names

RASSF1(Ras association (RalGDS/AF-6) domain family 1),123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association (RalGDS/AF-6) domain family member 1

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human RASSF1A (1-340aa) purified from E. coli.

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

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ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained with ATGA0528 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate

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The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: RASSF1A Transfected 293T cell lysate