Human EGFR antibody

Cat No.
ATGA0548

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT6E3
UniProt No.
P00533
NCBI Accession No.
NP_005219
Alternative names
ERBB, ERBB1, Epidermal growth factor receptor, HER1, PIG61, epidermal growth factor receptor, Urogastrone, Proto-oncogene c-ErbB-1, Oncogene ERBB, Cell proliferation inducing protein 61, Cell growth inhibiting protein 40, Avian erythroblastic leukemia viral (verbb) oncogene homolog
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human EGFR (424-605aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of EGFR in HeLa cells. The cell was stained with ATGA0548 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Hacat cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.