Human AlphaA Crystallin/CRYAA antibody

Cat No.
ATGA0550

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
c9F2
UniProt No.
P02489
NCBI Accession No.
NP_000385
Alternative names
Heat shock protein beta-4, HspB4, Alpha-crystallin A chain, Heat shock protein beta 4, Acry-1, Alphacrystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, HSPB 4, ZonularCentral Nuclear Cataract
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human AlphaA Crystallin/CRYAA (1-173aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human AlphaA Crystallin/CRYAA protein
Lane 2.: Recombinant human AlphaB Crystallin/CRYAB protein
The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: AlphaA Crystallin/CRYAA Transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.