NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

c9F2

UniProt No.

P02489

NCBI Accession No.

NP_000385

Alternative names

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human AlphaA Crystallin/CRYAA (1-173aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human AlphaA Crystallin/CRYAA protein
Lane 2.: Recombinant human AlphaB Crystallin/CRYAB protein

Magnify

The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: AlphaA Crystallin/CRYAA Transfected 293T cell lysate