NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

AT2N6

UniProt No.

P36952

NCBI Accession No.

NP_002630

Alternative names

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serine (or cysteine) proteinaseinhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5, SERPINB5, PI5, Serine (orcysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor, clade B,member 5, Ovalbumin, PI-5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin),member 5, SERPINB5, Spi5, Spi7

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant Maspin (1-375aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of Serpin B5/Maspin in HeLa cells. The cell was stained with ATGA0575 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Serpin B5/Maspin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: A431 cell lysate