Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT29G4
- UniProt No.
- Q9UGN5
- NCBI Accession No.
- NP_005475
- Alternative names
- Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PARP2 (233-583aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0578 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: SW480 cell lysate
Lane 1.: SW480 cell lysate
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 1.: HeLa cell lysate
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: NIH3T3 cell lysate
Lane 1.: Raji cell lysate
Lane 1.: NIH3T3 cell lysate
Lane 1.: Raji cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0578 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.