NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-321aa

UniProt No.

P18031

NCBI Accession No.

NP_002818.1

Alternative names

Protein tyrosine phosphatase non-receptor type 1, PTPN1, PTP1B, PTP-1B, Protein Tyrosine Phosphatase 1B, EC 3.1.3.48, Protein-tyrosine phosphatase1B, Tyrosine-protein phosphatase non-receptor type 1, Protein tyrosine phosphatase non-receptor type 1, Protein Tyrosine Phosphatase Non Receptor Type-1

Product Specification

Molecular Weight

37.3 kDa (321aa)

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA1mM DTT, 20% glycerol

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 10,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Kimura K et al., Pyrroloquinoline quinone stimulates epithelial cell proliferation by activating epidermal growth factor receptor through redox cycling. (Free Radic Biol Med. 2012)
Maeda A et al., Safranal, a novel protein tyrosine phosphatase 1B inhibitor, activates insulin signaling in C2C12 myotubes and improves glucose tolerance in diabetic KK-Ay mice. (Mol Nutr Food Res. 2014)
Quang TH et al., Protein Tyrosine Phosphatase 1B Inhibitors from the Roots of Cudrania tricuspidata. (Molecules. 2015)
Naito Y et al., Bis(hinokitiolato)zinc complex ([Zn(hkt)2]) activates Akt/protein kinase B independent of insulin signal transduction. (J Biol Inorg Chem. 2016)
Cho KH et al., Isolation and structure determination of a new diketopiperazine dimer from marine-derived fungus Aspergillus sp. SF-5280. (Nat Prod Res. 2018)
Park JS et al., Furanoaustinol and 7-acetoxydehydroaustinol: new meroterpenoids from a marine-derived fungal strain Penicillium sp. SF-5497. (J Antibiot (Tokyo). 2018)
VK Thu et al., Protein tyrosine phosphatase 1B inhibitory triterpene glycosides from Mussaenda pilosissima Valeton. (Phytochemistry Letters. 2020)
Kim HJ et al., PTP1B Inhibitory Secondary Metabolites from an Antarctic Fungal Strain Acremonium sp. SF-7394. (Molecules. 2021)
Huong PTM et al., Diterpenoid and phenolic constituents from corn silk (Zea mays) with PTP1B inhibitory activity. (Nat Prod Res. 2023)
Tran Thi Hong H et al., Iridoids from the aerial parts of Adenosma caeruleum with their nitric oxide inhibitory effects. (J Mol Struct. 2024)
Thi Ly Pham et al., PTP1B Inhibitory Activity of Flavonoids from the Roots of Astragalus membranaceus Bunge. (Nat Prod Sci. 2025)