Recombinant human PP2C alpha /PPM1A protein

Cat No.
PPC3001

Size

QTY

List Price

20ug

USD 55


100ug

USD 170

500ug

USD 505

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
1-382aa
UniProt No.
P35813
NCBI Accession No.
NP_066283
Alternative names
Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, PDP1, PDP, PPM2C, PP2C alpha, Protein phosphatase 2C, PDP 1, EC 3.1.3.43, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, PPM1A, Protein phosphatase 1A (formerly 2C), ppm1a, FLJ42306, MGC9201, PP2CA, PP2Calpha, PP2C-alpha, protein phosphatase Mg2+/Mn2+ dependent 1A

Product Specification

Molecular Weight
46.6 kDa (418aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 10mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 2mM DTT, 1mM MnCl2, 20%glycerol
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 1,400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
Enzyme Activity,SDS-PAGE
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.