Recombinant human EGF protein

Cat No.
EGF0501

Size

QTY

List Price

20ug

USD 25


100ug

USD 80

500ug

USD 185

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
971-1023aa
UniProt No.
P01133
NCBI Accession No.
NP_001954
Alternative names
Epidermal growth factor, Pro-epidermal growth factor, urogastrone, Epidermal growth factor, EGF, epidermal growth factor (beta-urogastrone)

Product Specification

Molecular Weight
6.3 kDa (54aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range is less or equal to 0.1 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.