Recombinant human TNF-alpha protein

Cat No.
TNF0501

Size

QTY

List Price

20ug

USD 55


100ug

USD 170

500ug

USD 505

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
77-233aa
UniProt No.
P01375
NCBI Accession No.
NP_000585.2
Alternative names
Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor ), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2,

Product Specification

Molecular Weight
17.5 kDa (158aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

Related Publications

    Li W, et al. Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. Biomol Ther (Seoul). 2014 Jul;22(4):334-40.
    Sun YN, et al. Nuclear Factor kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. Pharmacogn Mag. Jan-Mar 2016;12(45):31-5.

    Cho K, et al. Inhibition of TNF-a-Mediated NF-kB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and Sk-Hep1 Cells. Biomol Ther (Seoul). 2014 Jan;22(1):55-61.

    Thao NP, et al. Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. Arch Pharm Res. 2014 Jun;37(6):706-12.

    He F, et al. Antitumor effects of dammarane-type saponins from steamed Notoginseng. Pharmacogn Mag. 2014 Jul;10(39):314-7.

    Nhiem NX, et al. Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. J Med Food. 2012 Apr;15(4):369-77.

    Liang C, et al. Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. J Ginseng Res. 2013 Mar;37(1):74-9.

    Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. Biomol Ther (Seoul). 2014 Jan;22(1):55-61.

    Kwak MS, et al. Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli. Redox Biol. 2019 Jun;24:101203.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.