Recombinant human VEGF 121/VEGFA protein

Cat No.
ATGP0282

Size

QTY

List Price

20ug

USD 80


100ug

USD 240

500ug

USD 720

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
207-327aa
UniProt No.
P15692
NCBI Accession No.
NP_001020541.2
Alternative names
Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

Product Specification

Molecular Weight
14.2 kDa (122aa) confirmed by MALDI-TOF
Concentration
0.5mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.