NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-434aa

UniProt No.

P06733

NCBI Accession No.

NP_001419.1

Alternative names

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, PPH, Plasminogen-binding protein, ENO1L1, MBPB1, MPB1, ENO1-IT1, ENO1 intronic transcript 1

Product Specification

Molecular Weight

47.1 kDa (434aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Chen X, et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. (Oncotarget. 2017)
Hashimoto Y, et al. Comparison of clinical characteristics in patients with Vogt-Koyanagi-Harada disease with and without anti-retinal antibodies. (Graefes Arch Clin Exp Ophthalmol. 2019)
Hirakata T, et al. Acute unilateral inner retinal dysfunction with photophobia: importance of electrodiagnosis. (Jpn J Ophthalmol. 2020)
Hashimoto Y, et al. Comparison of clinical characteristics in patients with acute zonal occult outer retinopathy according to anti-retinal antibody status. (Graefes Arch Clin Exp Ophthalmol. 2021)
Inoue Y, et al. α-Enolase reduces cerebrovascular Aβ deposits by protecting Aβ amyloid formation. (Cell Mol Life Sci. 2022)