Recombinant human LTA/TNF-beta protein

Cat No.
ATGP0517

Size

QTY

List Price

10ug

USD 105


50ug

USD 320

250ug

USD 960

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
35-205aa
UniProt No.
P01374
NCBI Accession No.
NP_001153212
Alternative names
Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

Product Specification

Molecular Weight
20.9 kDa (192aa) confirmed by MALDI-TOF
Concentration
0.25mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.