Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-330aa
- UniProt No.
- P62136
- NCBI Accession No.
- NP_002699.1
- Alternative names
- Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A
Product Specification
- Molecular Weight
- 39.7 kDa (350aa) confirmed by MALDI-TOF
- Concentration
- 0.2mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol
- Purity
- > 80% by SDS-PAGE
- Bioactivity
- Specific activity: >3,000unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.