Recombinant human PP1 Catalytic protein

Cat No.
ATGP1174

Size

QTY

List Price

10ug

USD 240


50ug

USD 720

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
1-330aa
UniProt No.
P62136
NCBI Accession No.
NP_002699.1
Alternative names
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A

Product Specification

Molecular Weight
39.7 kDa (350aa) confirmed by MALDI-TOF
Concentration
0.2mg/ml (determined by Bradford assay)
Formulation
Liquid in. 50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol
Purity
> 80% by SDS-PAGE
Bioactivity
Specific activity: >3,000unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.