Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 21-645aa
- UniProt No.
- P13667
- NCBI Accession No.
- NP_004902.1
- Alternative names
- Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72
Product Specification
- Molecular Weight
- 72.9 kDa (646aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl,1mM DTT and 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.