Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-329aa
- UniProt No.
- P52643
- NCBI Accession No.
- NP_415898.1
- Alternative names
- NAD-dependent, JW1375, htpH, hslI, hslF, Fermentative D-lactate dehydrogenase, ECK1377
Product Specification
- Molecular Weight
- 39.1 kDa (353aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100mM NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.