Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-152aa
- UniProt No.
- P15531
- NCBI Accession No.
- NP_000260.1
- Alternative names
- NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23
Product Specification
- Molecular Weight
- 17.1 kDa (152aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 1,200unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.