Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-197aa
- UniProt No.
- Q16774
- NCBI Accession No.
- NP_000849
- Alternative names
- Guanylate kinase, GMK, GMP kinase, GMPK
Product Specification
- Molecular Weight
- 23.9 kDa (217aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is >100unit/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.