Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-434aa
- UniProt No.
- P17182
- NCBI Accession No.
- NP_075608
- Alternative names
- 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, c-Myc promoter binding protein
Product Specification
- Molecular Weight
- 49.5 kDa (457aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 6,000pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.