Recombinant rat TNF-alpha protein

Cat No.
ATGP3736

Size

QTY

List Price

10ug

USD 45


20ug

USD 105

50ug

USD 242

100ug

USD 320

250ug

USD 600

500ug

USD 1,020

1mg

USD 1,700

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
80-235aa
UniProt No.
P16599
NCBI Accession No.
NP_036807
Alternative names
Tumor necrosis factor, RATTNF, TNF-alpha, Tnfa

Product Specification

Molecular Weight
19.9 kDa (181aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤0.2ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE,Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..

Biological Activity

Rat TNF-alpha stimulates cytotoxicity of the L929 mouse fibroblast cells. The ED50 range ≤0.2 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.