Product Information
- Product Type
- Recombinant Protein
- Expression System
- HEK293
- Domain
- 24-99aa
- UniProt No.
- P13500
- NCBI Accession No.
- NP_002973.1
- Alternative names
- SMC-CF, Small inducible cytokine subfamily A member 2, Small inducible cytokine A2, SCYA2, Monocyte secretory protein JE, Monocyte chemotactic protein 1 homologous to mouse Sig-je, Monocyte chemotactic and activating factor, MGC9434, MCP-1, MCP1, MCAF, HC11, GDCF-2, C-C motif chemokine ligand 2
Product Specification
- Molecular Weight
- 9.5kDa (82aa)
- Concentration
- 0.25mg/ml (determined by Absorbance at 280nm)
- Formulation
- Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS - PAGE
- Bioactivity
- Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human CCL2/MCP-1 chemoattracts the the THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.