Product Information
- Product Type
- Recombinant Protein
- Expression System
- HEK293
- Domain
- 100-330aa
- UniProt No.
- P01857
- NCBI Accession No.
- CAC20454.1
- Alternative names
- immunoglobulin heavy chain constant region gamma 1, IGHG1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE
Product Specification
- Molecular Weight
- 25.9kDa (231aa)
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml. 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116). 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human Fc gamma RIIIA/CD16a (CAT# ATGP4102) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 1.5 ug/ml.
Human IgG1 Fc is coated at 1ug/ml (100 ul/well) can bind Human Fc gamma RIIA/CD32a (H167) (CAT# ATGP4116) in a Functional ELISA assay.
Human Fc gamma RIIIA/CD16a (CAT# ATGP4098) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 4 ug/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.