Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT3A2
- UniProt No.
- P31153
- NCBI Accession No.
- NP_005902
- Alternative names
- Methionine adenosyltransferase II alpha, MATA2, MATII, SAMS2, Methionine adenosyltransferase II alpha, MAT2A, Methionine adenosyltransferase II, alpha AdoMet synthetase, AdoMet synthetase 2, AMS 2, MAT II, MATA 2, Methionine adenosyltransferase, Methionine adenosyltransferase 2, SAMS 2, Methionine adenosyltransferase II, S adenosylmethionine synthetase gamma form, S adenosylmethionine synthetase isoform type 2, S-adenosylmethionine synthetase isoform type 2
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human MAT2A (1-395aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, IHC
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of MAT2A in HeLa cells. The cell was stained with ATGA0138 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Ramos cell lysate
Lane 3.: 293T cell lysate
Lane 4.: Jurkat cell lysate
Lane 5.: NIH-3T3 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: Ramos cell lysate
Lane 3.: 293T cell lysate
Lane 4.: Jurkat cell lysate
Lane 5.: NIH-3T3 cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.