NKMAXBio

DNA Cloning Servicee

One mutation is defined as any combination of mutation within a 3-base frame. Our delivery package includes vector containing target gene, complete sequencing data and construct maps. Prices vary according to the length of genes. If the target gene and destination vector isn’t provided by the customer, there will be additional charges. For specific pricing information, please contact us.

**DNA Cloning Service Table**
Service, Deliverable, Description, Price, Timeline information.
Service	Deliverable	Description	Price	Timeline
Subcloning (E.Coli)	Target gene inserted in destination vector that the customer provides	Sub cloning into one expression vector	$540	2 weeks
Subcloning (Baculovirus)	Target gene inserted in Baculovirus expression DNA (bacmid)	Sub cloning into one expression vector	$700	2-3 weeks
Additional service Mutagenesis	Target gene inserted in destination vector after mutagenesis	The first amino acid mutation (3 base pairs) is a fixed price in addition to the sub cloning fee. Please ask for a quote since different factors play a role in the price.	$540~	3 weeks

Notes:

1.The above price list is only for genes shorter than 1.5Kb For longer genes, we add an additional $115 per Kb for screening and sequencing.
2.The target gene must be provided by customer. If not, we charge $1,300 per gene (in addition to the sub cloning fee) and project duration will take 8 weeks instead of the above mentioned 2 weeks. If a specific destination vector is desired, the customer has to provide it. If DNA Synthesis is required, the price is $0.50/1bp. May be subject to change, so please email us for a price quote.
3.One mutation is defined as any combination of mutation within a 3-base frame.
4.Our delivery package includes the vector containing target gene and complete sequencing data and construct maps.

Protein Expression & Purification Service

NKMAX has a great expertise in developing recombinant proteins using Escherchia coli as a host. Our Protein services are charged according to the required amount of strain comparison and optimization work. The pricing is based on a setup fee for expression analysis, the number of different strains (possibly with different helper plasmid systems) and the number of different culture conditions tested. For specific pricing information, please contact our sales department.

E.Coli Expression Full Service

**Table**
Service, Deliverable, Description, Price, Timeline information.
Service	Deliverable	Description	Price	Timeline
*Expression and Identification of target protein	Report including SDS page image, expression levels according to E.coli strains, etc.	*Non-reimbursable setup charge to determine solubility and level of difficulty.	$100	1 week
Option 1 Purification	1L expression and 1-step purification.	Purified soluble tagged protein and prices vary according to the expression patterns.	$1,800~	2-3 weeks
Option 2 Non-Purification (Denatured)	Preparation of protein with inclusion body in buffer.	A denatured form of protein (without using conventional chromatography techniques) is provided.	$1,200	2-3 weeks
Additional service QC by WB (Ab provided by the customer)	Western blot assay image.	WB performed at no extra charge, but the customer has to provide the antibody for the target protein.	free	1 week
Additional service Endotoxin detection	Report	Whether the endotoxin level of target protein is above or below 1.0 EU per 1 microgram of protein is determined by LAL method. There is no guarantee that the level is < 1.0 EU per 1 microgram.	$150	1 week

* Expression and Identification of target protein is a mandatory first step to determine the price of purification. This step is paid in advance and is not refundable.

** Prices for scale up and additional purification steps (such as removing endotoxin, increase purity, refolding, specific buffer changes, concentration changes, etc.) are to be determined after a 1L pilot expression and 1-step purification.

*** If both client and NKMAX agree for NKMAX to add target protein to NKMAX’s product list, NKMAX will offer a 30% discount.

Baculovirus Expression Full Service

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Service, Deliverable, Description, Price, Timeline information.
Service	Deliverable	Description	Price	Timeline
Recombinant virus generation, protein expression, and purification	Report	*Non-reimbursable setup charge to determine feasibility.	$200	4-6 weeks
	Purified proteins and QC data.	Transfection of insect cell with recombinant Bacmid DNA.	$4,100	4-6 weeks
		Generation of P1 stock (Low titer), P2 stock (High titer)
		Insect cell expression, Infection of insect cells with P2 stock (5ml)
		Purification (expression in 200ml media with 1-step purification)

* Recombinant virus generation, protein expression, and purification is a mandatory step to determine the price of purification. This step is paid in advance and is not refundable.

** Prices for scale up and additional purification steps (such as removing endotoxin, increase purity, refolding, specific buffer changes, concentration changes, etc.) are to be determined after a 200mL pilot expression and 1-step purification.