NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

1B3

UniProt No.

O43464

NCBI Accession No.

NP_037379

Alternative names

HtrA serine peptidase 2, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, PRSS25, OMI, PARK13

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human HtrA2/Omi (134-458aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The cell lysates of 293T, MCF7, HT-29, HL-60, HeLa and tissue lysates of mouse brain, rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Related Publications

Kim GY, et al. A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease. (Biotechnol Lett. 2008)
Park HJ, et al. Intracellular Abeta and C99 aggregates induce mitochondria-dependent cell death in human neuroglioma H4 cells through recruitment of the 20S proteasome subunits. (Brain Res. 2009)
Shin EJ, et al. Matrix metalloproteinase-3 is activated by HtrA2/Omi in dopaminergic cells: relevance to Parkinsons disease. (Neurochem Int. 2012)
Goo HG, et al. HtrA2/Omi deficiency causes damage and mutation of mitochondrial DNA. (Biochim Biophys Acta. 2013)
Goo HG, et al. HtrA2/Omi influences the stability of LON protease 1 and prohibitin, proteins involved in mitochondrial homeostasis. (Exp Cell Res. 2014)
Nam MK, et al. HtrA2 regulates α-Synuclein-mediated mitochondrial reactive oxygen species production in the mitochondria of microglia. (Biochem Biophys Res Commun. 2023)