NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

3D9

UniProt No.

P09936

NCBI Accession No.

NP_004172

Alternative names

Ubiquitin C-terminal hydrolase L1, Ubiquitin carboxyl-terminal esterase L1, Ubiquitin thiolesterase L1, Parkinson Disease 5, PARK5, PGP9.5, Uch-L1, UCHL-1, Neuron cytoplasmic protein 9.5

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human PGP9.5 (1-223aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, IHC

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of PGP9.5 in U87MG cells. The cell was stained with APG0714 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain tissue lysate
Lane 2.: U87MG cell lysate

Immunohistochemistry (IHC)

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Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).