Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 4F12
- UniProt No.
- Q15116
- NCBI Accession No.
- AAH74740
- Alternative names
- Programmed cell death 1, CD279, PD1, hPD-1, hPD-l, PDCD1
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PDCD1 (21-167aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF,FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PD-1 in HeLa cells. The cell was stained with APD0811 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PD-1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Raji cell lysate
Lane 1.: 293T cell lysate
Lane 2.: Raji cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of PD-1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.