NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

k2E2

UniProt No.

P23284

NCBI Accession No.

NP_000933

Alternative names

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Cyclophilin B (26-216aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2
Lane 2.: HeLa

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: K562 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: A549 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: SK-OV-3 cell lysate
Lane 7.: LnCap cell lysate

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The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Cyclophilin B recombinant protein
Lane 2.: Cyclophilin A recombinant protein
Lane 3.: Cyclophilin D recombinant protein
Lane 4.: Cyclophilin E recombinant protein
Lane 5.: Cyclophilin F recombinant protein
Lane 6.: Cyclophilin G recombinant protein
Lane 7.: Cyclophilin H recombinant protein

Flow cytometry (FACS)

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Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Related Publications

Choi JW, et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. (PLoS Genet. 2009)
Dubnikov T, et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. (J Cell Sci. 2016)
Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018)