NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

J4H9

UniProt No.

P05067

NCBI Accession No.

NP_000475

Alternative names

amyloid beta A4 protein precursor, isoform, amyloid beta (A4) precursor protein, Alzheimer disease, peptidase nexin-II

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human APP (18-289aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAP0836 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate

Flow cytometry (FACS)

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Flow cytometry analysis of APP/Protease Nexin II in 293T cells. The cell was stained with AAP0836 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).