NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT18F6

UniProt No.

P07711

NCBI Accession No.

NP_001244901

Alternative names

Procathepsin L, Cathepsin L1, Major excreted protein, MEP, CTSL, CTSL1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human CTSL (18-333aa) purified from E. coli.

Isotype

IgG2a kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of CTSL in A549 cells. The cell was stained with ACT0905 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate

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The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: CTSB recombinant protein
Lane 2.: CTSD recombinant protein
Lane 3.: CTSE recombinant protein
Lane 4.: CTSF recombinant protein
Lane 5.: CTSH recombinant protein
Lane 6.: CTSK recombinant protein
Lane 7.: CTSL recombinant protein
Lane 8.: CTSS recombinant protein
Lane 9.: CTSW recombinant protein
Lane 10.: CTSZ recombinant protein

Flow cytometry (FACS)

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Flow cytometry analysis of CTSL in A549 cells. The cell was stained with ACT0905 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).