Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 2G4
- UniProt No.
- P07148
- NCBI Accession No.
- NP_001434
- Alternative names
- Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human FABP1 (1-127aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, IHC, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: FABP1 Recombinant protein
Lane 2.: HepG2 cell lysate
Lane 3.: Liver tissues lysate
Lane 1.: FABP1 Recombinant protein
Lane 2.: HepG2 cell lysate
Lane 3.: Liver tissues lysate
Flow cytometry (FACS)
Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Immunohistochemistry (IHC)
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.