Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 3A6
- UniProt No.
- P56704
- NCBI Accession No.
- NP_149122
- Alternative names
- Wnt3a, Wingless-type MMTV integration site family, member 3A
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Wnt3a (19-352aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, IHC
- Usage
- The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human
Wnt3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Wnt3a Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: Wnt3a Transfected 293T cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.