Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT2G5
- UniProt No.
- P42212
- Alternative names
- Green fluorescent protein
Product Specification
- Host
- Mouse
- Reacts With
- Aequorea victoria
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant GFP (1-238aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB
- Usage
- The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The recombinant proteins were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human GFP protein (20ng)
Lane 2.: Recombinant human GFP protein (40ng)
Lane 3.: Recombinant human GFP protein (80ng)
Lane 1.: Recombinant human GFP protein (20ng)
Lane 2.: Recombinant human GFP protein (40ng)
Lane 3.: Recombinant human GFP protein (80ng)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: GFP transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: GFP transfected 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.