Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1A1
- UniProt No.
- P29762
- NCBI Accession No.
- NP_004369
- Alternative names
- Cellular retinoic acid binding protein 1, CRABP, CRABP-I, CRABPI, RBP5, Cellular retinoic acid binding protein 1 CRABP 1, CRABP I, RBP 5, Retinoic acid binding protein I cellular
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human CRABP1 (1-137aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of CRABP1 in Balb/3T3 cells. The cell was stained with ATGA0162 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The tissue lysate(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Eye Tissue lysate
Lane 1.: Mouse Eye Tissue lysate
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: MCF7 cell lysate
Lane 1.: MCF7 cell lysate
The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: CRABP1 Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: CRABP1 Transfected 293T cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.