NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT1E3

UniProt No.

P16083

NCBI Accession No.

AAH06096

Alternative names

N-ribosyldihydronicotinamide:quinone reductase 2, NMOR2, NAD(P)H menadione oxidoreductase 2, Dioxin-inducible, NAD(P)H quinone dehydrogenase 2, Quinone reductase 2, QR2, DHQV, DIA6, NRH:quinone oxidoreductase 2

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human NQO2 (1-231aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of NQO2 in A549 cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

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ICC/IF analysis of NQO2 in HeLa cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: A549 cell lysate