Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT6D10
- UniProt No.
- Q04828
- NCBI Accession No.
- NP_001344
- Alternative names
- Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human AKR1C1 (1-323aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of AKR1C1 in A431 cells. The cell was stained with ATGA0201 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human AKR1C1
Lane 2. : HepG2 cell lysate
Lane 3. : Raji cell lysate
Lane 1. : Recombinant Human AKR1C1
Lane 2. : HepG2 cell lysate
Lane 3. : Raji cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of AKR1C1 in A431 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.