Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1B3
- UniProt No.
- P84077
- NCBI Accession No.
- NP_001649
- Alternative names
- ADP-ribosylation factor 1, ADP ribosylation factor 1
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human ARF1 (1-181aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF,FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of ARF1 in MCF7 cells. The cell was stained with ATGA0207 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of ARF1 in U87MG cells. The cell was stained with ATGA0207 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: Brain tissue lysate
Lane 4.: MCF cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: Brain tissue lysate
Lane 4.: MCF cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of ARF1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.