Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT2H8
- UniProt No.
- P00533
- NCBI Accession No.
- NP_005219
- Alternative names
- Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human EGFR (424-605aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green).
Western blot analysis (WB)
The cell lysates of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.