NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT4B9

UniProt No.

P61981

NCBI Accession No.

NP_036611

Alternative names

YWHAG, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma N-terminally processed, protein phosphatase 1, regulatory subunit 170, PPP1R170, 14-3-3γ

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

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The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human YWHAZ
Lane 2.: Recombinant Human YWHAB
Lane 3.: Recombinant Human YWHAE
Lane 4.: Recombinant Human YWHAH
Lane 5.: Recombinant Human YWHAG
Lane 6.: Recombinant Human SFN
Lane 7.: Recombinant Human YWHAQ

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: A431 cell lysate
Lane 3.: K562 cell lysate
Lane 4.: NIH3T3 cell lysate