NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT11D10

UniProt No.

P00390

NCBI Accession No.

NP_000628

Alternative names

Glutathione reductase, GLuR, GRD1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human GSR (43-522aa) purified from E.coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of GSR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

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The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human GSR protein

Flow cytometry (FACS)

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Flow cytometry analysis of GSR in Jurkat cells. The cell was stained with ATGA0323 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).