Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT14G8
- UniProt No.
- P78560
- NCBI Accession No.
- NP_003796
- Alternative names
- Caspase and RIP adapter with death domain, RAIDD, CRADD, Caspase and RIP adapter with death domain, Caspase and RIP adapter with death domain CASP2 and RIPK1 domain containing adaptor with death domain, Death adaptor molecule RAIDD, Death domain containing protein CRADD, MGC9163, RIP associated ICH1/CED3 homologous protein with death domain, RIP associated protein with a death domain.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human CRADD (1-199aa) purified from E. coli
- Isotype
- IgG1a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of CRADD in HeLa cells. The cell was stained with ATGA0327 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of CRADD in PC3 cells. The cell was stained with ATGA0327 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: A549 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: MCF-7 cell lysate
Lane 4.: 293T cell lysate
Lane 1.: A549 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: MCF-7 cell lysate
Lane 4.: 293T cell lysate
The Recombinant Human CRADD (50,100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Protein 50 ng
Lane 2.: Recombinant Protein 100 ng
Lane 1.: Recombinant Protein 50 ng
Lane 2.: Recombinant Protein 100 ng
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.