NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT5D1

UniProt No.

Q14749

NCBI Accession No.

NP_061833

Alternative names

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, Glycine N-methyltransferase EC 2.1.1.20, Glycine N methyltransferase

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human GNMT (1-295aa) purified from E. coli.

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of GNMT in HepG2 cells. The cell was stained with ATGA0370 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The recombinant protein (50ng) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human GNMT protein
Lane 2.: Mouse Liver tissue lysate

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The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: GNMT Transfected 293T cell lysate