Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1F8
- UniProt No.
- P30048
- NCBI Accession No.
- NP_006784
- Alternative names
- Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Peroxiredoxin3 (63-256aa) purified from E. coli
- Isotype
- IgG3 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Peroxiredoxin3 in HeLa cells. The cell was stained with ATGA0407 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human PRDX3 protein
Lane 2.: HeLa cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: TF1 cell lysate
Lane 5.: U87MG cell lysate
Lane 6.: Raji cell lysate
Lane 7.: 293T cell lysate
Lane 8.: Jurkat cell lysate
Lane 9.: MCF7 cell lysate
Lane 1.: Recombinant human PRDX3 protein
Lane 2.: HeLa cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: TF1 cell lysate
Lane 5.: U87MG cell lysate
Lane 6.: Raji cell lysate
Lane 7.: 293T cell lysate
Lane 8.: Jurkat cell lysate
Lane 9.: MCF7 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.