NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT4C12

UniProt No.

P52790

NCBI Accession No.

NP_002106

Alternative names

EC 2.7.1.1, Hexokinase 3, Hexokinase type III, HK III, HK3, HXK3

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Hexokinase1,2,3 (1-923aa) purified from E. coli.

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombianat protein (20ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant human Hexokinase 1
Lane 2 : Recombinant human Hexokinase 2
Lane 3 : Recombinant human Hexokinase 3
Lane 4 : Recombinant human Hexokinase 4

Magnify

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: K562 cell lysate
Lane 4.: Mouse brain tissue lysate