NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

AT23F1

UniProt No.

P06748

NCBI Accession No.

NP_002511

Alternative names

Nucleophosmin, B23, NPM

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human NPM1(1-294aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of NPM1 in HeLa cells. The cell was stained with ATGA0565 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 1.: Jurkat cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of NPM1 in WiDr cells. The cell was stained with ATGA0565 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).