Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 4D7
- UniProt No.
- P19367
- NCBI Accession No.
- NP_000179
- Alternative names
- BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase-A, HK I, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HXK1, mHk1-s, tumor isozyme, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase 4, HK1,HK2, HK3, HK4
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Hexokinase1 (1-917aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained with ATGA0569 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human Hexokinase1 protein
Lane 2.: Recombinant human Hexokinase2 protein
Lane 3.: Recombinant human Hexokinase3 protein
Lane 4.: Recombinant human Hexokinase4 protein
Lane 1.: Recombinant human Hexokinase1 protein
Lane 2.: Recombinant human Hexokinase2 protein
Lane 3.: Recombinant human Hexokinase3 protein
Lane 4.: Recombinant human Hexokinase4 protein
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: K562 cell lysate
Lane 5.: Mouse brain Tissue lysate
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: K562 cell lysate
Lane 5.: Mouse brain Tissue lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.